A functional ribonucleoprotein complex forms around the 5′ end of poliovirus RNA
Abstract
The existence of a computer-predicted cloverleaf structure for the first 100 nucleotides at the 5′ end of poliovirus RNA was verified by site-directed mutagenesis and by chemical and RNAase probing. Mutations that modified the cloverleaf in the positive strand but not the negative strand were lethal to the virus. This RNA cloverleaf structure binds a cellular protein and the viral proteins 3Cᵖʳᵒ and 3Dᵖᵒⁱ. Mutations in specific regions of the RNA cloverleaf prevented this binding. Mutations in either 3Cᵖʳᵒ or the RNA that disrupted ribonucleoprotein complex formation inhibited virus growth and selectively affected positive strand RNA accumulation. Phenotypic reversion of these mutations restored the ability to form the complex. Thus, a cloverleaf structure in poliovirus RNA plays a central role in organizing viral and cellular proteins involved in positive strand production.
Additional Information
© 1990 Cell Press. Received 16 May 1990, Revised 2 August 1990. We are grateful to Drs. Nahum Sonenberg and Douglas Black for technical advice, Cristina Giachetti for anti-3C antiserum, Gerardo Kaplan and Cara Burns for anti-3D antiserum, and Douglas Black, Xiao-Hong Sun, Mark Muesing, and Mark Feinberg for their useful comments on the manuscript. This work was supported by Public Health Service grant #AI 22346 to D. B. R. A. is recipient of a fellowship from the Consejo National de Investigaciones Cientificas y Tecnicas-Argentina. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.Additional details
- Eprint ID
- 103888
- DOI
- 10.1016/0092-8674(90)90170-j
- Resolver ID
- CaltechAUTHORS:20200612-135406435
- NIH
- AI 22346
- Consejo National de Investigaciones Cientificas y Tecnicas (Argentina)
- Created
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2020-06-12Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field