Ethidium analogues with improved resolution in the dye-buoyant density procedure
Abstract
Analogues of ethidium chloride with large quaternary side chains have been synthesized and evaluated for improved resolution in the dye-buoyant density system for the separation of open and closed circular DNA. These compounds are similar to propidium which differs from ethidium by the replacement of an ethyl group by a methyldiethylaminopropyl group. The new analogues contain a triethylamino group attached to a methylene chain with 3, 5 or 7 carbons. With PM2 DNA the observed separation for propidium is 1.97 times that of ethidium and the new dyes show separations relative to ethidium of 2.27, 2.66 and 2.77. A correlation is established between the mass of the dye component and the observed separation which is rationalized on the basis of the four component thermodynamics describing this system.
Additional Information
© 1977 Information Retrieval Limited. Received 12 January 1977. This work was supported by a grant from the Research Corporation and U. S. Public Health Service grant CA15461. B. H. is a Camille and Henry Dreyfus Teacher-Scholar and an Alfred P. Sloan Foundation Fellow. We wish to thank Eric Rothenberg who prepared the PM2 phage.Attached Files
Published - nar00478-0193.pdf
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Additional details
- PMCID
- PMC343760
- Eprint ID
- 103762
- Resolver ID
- CaltechAUTHORS:20200608-115353955
- Research Corporation
- CA15461
- NIH
- Camille and Henry Dreyfus Foundation
- Alfred P. Sloan Foundation
- Created
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2020-06-08Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field