Rel-associated pp40: an inhibitor of the rel family of transcription factors
Abstract
The Rel-associated protein pp40 is functionally related to IκB, an inhibitor of the transcription factor NF-κB. Purified pp40 inhibits the DNA binding activity of the NF-κB protein complex (p50:p65 heterodimers), p50:c-Rel heteromers, and c-Rel homodimers. The sequence of the complementary DNA encoding pp40 revealed similarity to the gene encoding MAD-3, a protein with mammalian IκB-like activity. Protein sequencing of IκB purified from rabbit lung confirmed that MAD-3 encodes a protein similar to IκB. The sequence similarity between MAD-3 and pp40 includes a casein kinase II and consensus tyrosine phosphorylation site, as well as five repeats of a sequence found in the human erythrocyte protein ankyrin. These results suggest that rel-related transcription factors, which are capable of cytosolic to nuclear translocation, may be held in the cytosol by interaction with related cytoplasmic anchor molecules.
Additional Information
© 1991 American Association for the Advancement of Science. Received 3 July 1991; accepted 31 July 1991. The first two authors contributed equally to this work. We thank I. Verma for the c-rel done, M. Gifford for excellent technical help, M. Schlissel, M. Feinberg, and E. Spanopoulou for reviewing the manuscript, and X.-H. Sun for help and stimulating discussions. Supported by an NIH training grant CA09583 (N.D.), postdoctoral fellowships from Irvington Institute for Medical Research (S.G.) and the Cancer Research Institute (H.C.L.), NIH grants Ca33192 and Ca2616 (D.B. and H.R.B.) and the Bireley Foundation (D.S.)Additional details
- Eprint ID
- 103219
- Resolver ID
- CaltechAUTHORS:20200514-161356013
- NIH
- CA09583
- Irvington Institute for Medical Research
- Cancer Research Institute
- NIH
- CA33192
- NIH
- CA2616
- Bireley Foundation
- Created
-
2020-05-14Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field