Identification of a protein that binds to the SH3 region of Abl and is similar to Bcr and GAP-rho
Abstract
A Src homology 3 (SH3) region is a sequence of approximately 50 amino acids found in many nonreceptor tyrosine kinases and other proteins. Deletion of the SH3 region from the protein encoded by the c-abl proto-oncogene activates the protein's transforming capacity, thereby suggesting the participation of the SH3 region in the negative regulation of transformation. A complementary DNA was isolated that encoded a protein, 3BP-1, to which the SH3 region of Abl bound with high specificity and to which SH3 regions from other proteins bound differentially. The sequence of the 3BP-1 protein is similar to that of a COOH-terminal segment of Bcr and to guanosine triphosphatase-activating protein (GAP)-rho, which suggests that it might have GAP activity for Ras-related proteins. The 3BP-1 protein may therefore be a mediator of SH3 function in transformation inhibition and may link tyrosine kinases to Ras-related proteins.
Additional Information
© 1992 American Association for the Advancement of Science. Received 22 April 1992; accepted 16 June 1992. The λgt11 library was the kind gift of A. Winoto. We thank P. K. Jackson for helpful discussions and the c-abl SH3-GST fusion probe, R. Darnell for mouse brain mRNA, A. Ray for helpful advice and the Y1089 bacterial strain, and E. Schaeffer for bacterial strain Y1090. Supported by National Research Service award training grant A107233 (P.C.), B. Duke Glenn Fellowship (P.C.), National Research Service award CA 08875 (B.J.M.), and U.S. Public Health Service grant CA 51462 (D.B.).Additional details
- Eprint ID
- 103212
- DOI
- 10.1126/science.1379745
- Resolver ID
- CaltechAUTHORS:20200514-150240015
- A107233
- NIH Predoctoral Fellowship
- B. Duke Glenn Fellowship
- CA08875
- NIH
- CA51462
- NIH
- Created
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2020-05-14Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field