Acoustically Targeted Chemogenetics for Noninvasive Control of Neural Circuits
Abstract
Existing treatments for brain disorders aim to modulate the activity of neural circuits, but are either not cell type-specific, lack spatial targeting, or require invasive procedures. Previously, we introduced an approach to modulating neural circuits noninvasively with spatial, cell-type, and temporal specificity called acoustically targeted chemogenetics, or ATAC. We use ultrasound to open the blood brain barrier to transduce neurons at specific locations in the brain with virally-encoded chemogenetic receptors. To show neuronal inhibition expressed inhibitory DREADD (hM4Di) throughout the hippocampus and tested mice in a fear conditioning protocol. The context fear test showed that mice treated with saline froze significantly more than those who had received CNO (p<2E-5, n=7,11) during the training phase.
Additional Information
© 2020 Published by Elsevier Inc. Supported By: NARSAD, Jacobs Institute for Molecular Engineering for Medicine, DARPA.Additional details
- Eprint ID
- 103074
- DOI
- 10.1016/j.biopsych.2020.02.263
- Resolver ID
- CaltechAUTHORS:20200507-125032304
- Brain and Behavior Research Foundation
- Jacobs Institute for Molecular Engineering for Medicine
- Defense Advanced Research Projects Agency (DARPA)
- Created
-
2020-05-07Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field
- Caltech groups
- Jacobs Institute for Molecular Engineering for Medicine