Deletion of the Igκ Light Chain Intronic Enhancer/Matrix Attachment Region Impairs but Does Not Abolish VκJκ Rearrangement
Abstract
Roles of the κ intronic enhancer (iEκ) and its asssociated matrix attachment region (MAR) during B cell development were examined using mutant embryonic stem (ES) cell lines in which the entire region on both chromosomes was replaced with either a recombined LoxP site (EκND) or the PGK–neomycin resistance (PGK-neor) gene (EκNI). B cells derived from EκND ES cells had greatly impaired VκJκ rearrangement, normal levels of κ expression, and κ:λ ratios of 1:1 instead of the usual 10:1. Furthermore, λ-producing hybridomas derived from EκND cells displayed little κ rearrangement. Thus, the MAR and iEκ are quantitatively significant for κ rearrangement but not necessary. In addition, little VκJκ rearrangement could be detected in B cells derived from EκNI ES cells, demonstrating that an inserted PGK–neor gene dominantly suppresses VκJκ rearrangement.
Additional Information
© 1996 Cell Press. Received 18 January 1996, Revised 13 March 1996. Correspondence should be addressed to D. B. We thank Dr. E. Selsing for the RS probe; S. Cherry and Dr. C. Roman for critically reading the manuscript. This work was supported by a National Institutes of Health grant to D. B., National Institutes of Health grant AI-20047 to F. W. A., and the Howard Hughes Medical Institute. Y. X. was supported by a fellowship from the Cancer Research Fund of the Damon Runyon–Walter Winchell Foundation. D. B. is an American Cancer Society Research Professor.Additional details
- Eprint ID
- 103072
- Resolver ID
- CaltechAUTHORS:20200507-112119157
- AI-20047
- NIH
- Howard Hughes Medical Institute (HHMI)
- Damon Runyon Cancer Research Foundation
- American Cancer Society
- Created
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2020-05-08Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field