Developmental expression of prion protein gene in brain
Abstract
Synthesis of the cellular isoform of the prion protein (PrP^C) was found to be regulated during development of the hamster brain. PrP poly A(+) RNA was readily detectable 10 days postpartum; after 20 days of age, no change in its level could be detected through 13 months of age. Low levels of PrP poly A(+) RNA were detectable 1 day after birth. By contrast, myelin basic protein poly A(+) RNA was found at high levels in brain at 30 days of age and thereafter declined steadily. Using monospecific PrP antisera, immunoprecipitable cell-free translation products were detected at low levels 2 days after birth and increased progressively through 10 days of age. How the levels of PrP mRNA participate in brain development and function remains to be established.
Additional Information
© 1987 Published by Elsevier Inc. Received 16 May 1986, Accepted 13 October 1986. We thank Dr. David Westaway for advice on the RNA analysis and for critical review of the manuscript, Dr. Karen Hsiao for helpful discussions, and Lorraine Gallagher for manuscript preparation. This work was supported by research grants from the National Institutes of Health (AG02132 and NS14069) and a Senator Jacob Javits Center of Excellence in Neuroscience, as well as by gifts from RJR-Nabisco, Inc., and Sherman Fairchild Foundation.Additional details
- Eprint ID
- 102899
- DOI
- 10.1016/0012-1606(87)90143-6
- Resolver ID
- CaltechAUTHORS:20200429-083757573
- AG02132
- NIH
- NS14069
- NIH
- RJR-Nabisco
- Sherman Fairchild Foundation
- Created
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2020-04-29Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field