Unnatural amino acid mutagenesis in mapping ion channel function

Creators: Beene, Darren L.; Dougherty, Dennis A.; Lester, Henry A.

Abstract

Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure–function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.

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Resource type: Journal Article
Publisher: Elsevier
Published in: Current Opinion in Neurobiology, 13(3), 264-270, ISSN: 0959-4388.

Created:: August 19, 2023
Modified:: October 20, 2023