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Published December 1, 2000 | public
Journal Article

Unnatural amino acids as probes of protein structure and function

Abstract

Nonsense suppression methodology, for incorporating unnatural amino acids into proteins, has enabled a wide range of studies into protein structure and function using both in vitro and in vivo translation systems. Although methodological challenges remain, scores of unnatural amino acids have been employed that include both subtle and dramatic variants of the natural set. A number of insights that would not have been possible using conventional site-directed mutagenesis have been gained.

Additional Information

© 2000 Elsevier. Available online 27 November 2000. The work done at Caltech is the result of an enjoyable collaboration with my colleague Henry Lester, Division of Biology at Caltech. I gratefully acknowledge the experimental and intellectual contributions of the many outstanding graduate students and postdoctorates who have been part of the 'unnatural' group. Our work in this field has been supported by The National Institutes fo Health (NS-34407).

Additional details

Created:
August 21, 2023
Modified:
October 20, 2023