Control of convergent yolk syncytial layer nuclear movement in zebrafish
Abstract
Nuclear movements play an essential role in metazoan development. Although the intracellular transport mechanisms underlying nuclear movements have been studied in detail, relatively little is known about signals from surrounding cells and tissues controlling these movements. Here, we show that, in gastrulating zebrafish embryos, convergence movements of nuclei within the yolk syncytial layer (YSL) are guided by mesoderm and endoderm progenitors migrating along the surface of the yolk towards the dorsal side of the developing gastrula. Progenitor cells direct the convergence movements of internal yolk syncytial nuclei (iYSN) by modulating cortical flow within the YSL in which the iYSN are entrained. The effect of mesoderm and endoderm progenitors on the convergence movement of iYSN depends on the expression of E-cadherin, indicating that adhesive contact between the cells and the YSL is required for the mesendoderm-modulated YSL cortical flow mediating nuclear convergence. In summary, our data reveal a crucial function for cortical flow in the coordination of syncytial nuclear movements with surrounding cells and tissues during zebrafish gastrulation.
Additional Information
© 2009 Company of Biologists. Accepted February 11, 2009. We are grateful to M. Wilsch-Bräuninger for advice and assistance with electron microscopy, and to B. Flach for supervising the development of the image segmentation algorithm. We also thank J. Peychl for advice and assistance with confocal and two-photon excitation microscopy, S. Grill for advice and assistance with the fluid flow analysis, T. Pietzsch for advice with the Kalman Filter, and G. Junghanns, E. Lehmann and J. Hückmann for fish care. We thank M. Köppen, A. Oates, S. Saalfeld, G. Soete and P. Tomancak for critical reading of the manuscript. This work was supported by grants from the Human Frontier Science Program to J.S.B., and the Deutsche Forschungsgemeinschaft, European Community (ZF-Models, Endotrack, ZF-Cancer), and Max-Planck-Society to C.P.H.Attached Files
Published - 1305.full.pdf
Supplemental Material - 026922-figS1.jpg
Supplemental Material - 026922-figS2.jpg
Supplemental Material - 026922-figS3.jpg
Supplemental Material - 026922-figS4.jpg
Supplemental Material - 026922-movies_1-22.zip
Supplemental Material - DEV026922TableS1.pdf
Supplemental Material - DEV026922TableS2.pdf
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Additional details
- Eprint ID
- 102552
- Resolver ID
- CaltechAUTHORS:20200415-094705543
- Human Frontier Science Program
- Deutsche Forschungsgemeinschaft (DFG)
- European Community
- Max-Planck-Society
- Created
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2020-04-15Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field