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Published April 15, 2009 | Published + Supplemental Material
Journal Article Open

Control of convergent yolk syncytial layer nuclear movement in zebrafish

Abstract

Nuclear movements play an essential role in metazoan development. Although the intracellular transport mechanisms underlying nuclear movements have been studied in detail, relatively little is known about signals from surrounding cells and tissues controlling these movements. Here, we show that, in gastrulating zebrafish embryos, convergence movements of nuclei within the yolk syncytial layer (YSL) are guided by mesoderm and endoderm progenitors migrating along the surface of the yolk towards the dorsal side of the developing gastrula. Progenitor cells direct the convergence movements of internal yolk syncytial nuclei (iYSN) by modulating cortical flow within the YSL in which the iYSN are entrained. The effect of mesoderm and endoderm progenitors on the convergence movement of iYSN depends on the expression of E-cadherin, indicating that adhesive contact between the cells and the YSL is required for the mesendoderm-modulated YSL cortical flow mediating nuclear convergence. In summary, our data reveal a crucial function for cortical flow in the coordination of syncytial nuclear movements with surrounding cells and tissues during zebrafish gastrulation.

Additional Information

© 2009 Company of Biologists. Accepted February 11, 2009. We are grateful to M. Wilsch-Bräuninger for advice and assistance with electron microscopy, and to B. Flach for supervising the development of the image segmentation algorithm. We also thank J. Peychl for advice and assistance with confocal and two-photon excitation microscopy, S. Grill for advice and assistance with the fluid flow analysis, T. Pietzsch for advice with the Kalman Filter, and G. Junghanns, E. Lehmann and J. Hückmann for fish care. We thank M. Köppen, A. Oates, S. Saalfeld, G. Soete and P. Tomancak for critical reading of the manuscript. This work was supported by grants from the Human Frontier Science Program to J.S.B., and the Deutsche Forschungsgemeinschaft, European Community (ZF-Models, Endotrack, ZF-Cancer), and Max-Planck-Society to C.P.H.

Attached Files

Published - 1305.full.pdf

Supplemental Material - 026922-figS1.jpg

Supplemental Material - 026922-figS2.jpg

Supplemental Material - 026922-figS3.jpg

Supplemental Material - 026922-figS4.jpg

Supplemental Material - 026922-movies_1-22.zip

Supplemental Material - DEV026922TableS1.pdf

Supplemental Material - DEV026922TableS2.pdf

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Created:
August 20, 2023
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