Published July 30, 1999
| public
Journal Article
Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell Wall
Abstract
Surface proteins of Gram-positive bacteria are linked to the bacterial cell wall by a mechanism that involves cleavage of a conserved Leu-Pro-X-Thr-Gly (LPXTG) motif and that occurs during assembly of the peptidoglycan cell wall. A Staphylococcus aureus mutant defective in the anchoring of surface proteins was isolated and shown to carry a mutation in the srtA gene. Overexpression of srtA increased the rate of surface protein anchoring, and homologs of srtA were found in other pathogenic Gram-positive bacteria. The protein specified bysrtA, sortase, may be a useful target for the development of new antimicrobial drugs.
Additional Information
© 1999 American Association for the Advancement of Science. 6 May 1999; accepted 28 June 1999. We thank W. W. Navarre for help in determining the surface protein anchor structure of strain SM317, and D. Missiakas, P. Model, M. Russel, and members of our laboratory for critical reading of this manuscript. S.K.M. was supported by the Predoctoral Training Program in Genetic Mechanisms at UCLA (T32GM07104). H.T.-T. was supported by the Microbial Pathogenesis Training Grant at UCLA (AI 07323). Work in O.S.'s laboratory is supported by grant AI33987 from the National Institute of Allergy and Infectious Diseases.Additional details
- Eprint ID
- 101832
- DOI
- 10.1126/science.285.5428.760
- Resolver ID
- CaltechAUTHORS:20200310-142734417
- T32GM07104
- NIH Predoctoral Fellowship
- AI07323
- NIH Predoctoral Fellowship
- AI33987
- NIH
- Created
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2020-03-10Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field