Published September 2005
| public
Journal Article
A Dual Selection Module for Directed Evolution of Genetic Circuits
- Creators
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Yokobayashi, Yohei
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Arnold, Frances H.
Abstract
We have developed a genetic selection module which enables us to select for positive and negative expression of a gene by controlling the expression of two antibiotic resistance genes. This module can be used to tune the performance of genetic circuits that yield simple ON/OFF gene expression outputs depending on the environment (input). We have demonstrated the utility of the module by selecting a genetic inverter from a 200 fold excess of nonfunctional inverters in two rounds.
Additional Information
© 2005 Springer. We thank Tim Palzkill for providing pTP154 from which bli gene was engineered and the National Science Foundation (Award # 0130613) for financial support.Additional details
- Eprint ID
- 101461
- DOI
- 10.1007/s11047-004-7442-x
- Resolver ID
- CaltechAUTHORS:20200221-125714643
- CCF-0130613
- NSF
- Created
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2020-02-21Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field