Design and application of stationary phase combinatorial promoters
Abstract
Current bacterial synthetic circuits rely on the fast dilution and high protein expression that occurs during exponential phase. However, constant exponential phase is both difficult to ensure in a lab environment and almost certainly impractical in any natural setting. Here, we characterize the performance of 13 E. coli native σ38 promoters, as well as a previously identified σ38 consensus promoter. We then make tetO combinatorial versions of the three strongest promoters to allow for inducible delayed expression. The design of these combinatorial promoters allows for design of circuits with inducible stationary phase activity that can be used for phase-dependent delays in dynamic circuits or spatial partitioning of biofilms.
Additional Information
VH is supported by the Department of Defense (DoD) through the National Defense Science & Engineering Graduate Fellowship (NDSEG) Program. Research supported in part by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.Attached Files
Submitted - hcm16-seed_s.pdf
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Additional details
- Eprint ID
- 101129
- Resolver ID
- CaltechAUTHORS:20200205-075449007
- Department of Defense
- National Defense Science and Engineering Graduate (NDSEG) Fellowship
- Army Research Office (ARO)
- W911NF-09-0001
- Created
-
2020-02-06Created from EPrint's datestamp field
- Updated
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2020-02-06Created from EPrint's last_modified field
- Caltech groups
- Division of Biology and Biological Engineering (BBE)