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Published July 2020 | Submitted + Published + Supplemental Material
Journal Article Open

Vein patterning by tissue-specific auxin transport

Abstract

Unlike in animals, in plants, vein patterning does not rely on direct cell-cell interaction and cell migration; instead, it depends on the transport of the plant hormone auxin, which in turn depends on the activity of the PIN-FORMED1 (PIN1) auxin transporter. The current hypotheses of vein patterning by auxin transport propose that, in the epidermis of the developing leaf, PIN1-mediated auxin transport converges to peaks of auxin level. From those convergence points of epidermal PIN1 polarity, auxin would be transported in the inner tissues where it would give rise to major veins. Here, we have tested predictions of this hypothesis and have found them unsupported: epidermal PIN1 expression is neither required nor sufficient for auxin transport-dependent vein patterning, whereas inner-tissue PIN1 expression turns out to be both required and sufficient for auxin transport-dependent vein patterning. Our results refute all vein patterning hypotheses based on auxin transport from the epidermis and suggest alternatives for future tests.

Additional Information

© 2020 Published by The Company of Biologists Ltd. Received 20 December 2019; Accepted 27 May 2020; Published 6 July 2020. We thank the Arabidopsis Biological Resource Center for PIN1::gPIN1:CFP, PIN1 cDNA and pin1-1; the Nottingham Arabidopsis Stock Centre for pin1-051; Ikram Blilou and Ben Scheres for pin3-3, pin4-2 and pin7^(En); Megan Sawchuk for PIN1::nYFP; and Jian Xu and Ben Scheres for PIN1::gPIN1:YFP and PIN1::gPIN1:GFP. This work was supported by Discovery Grants of the Natural Sciences and Engineering Research Council of Canada [RGPIN-2016-04736 to E.S.]. C.V. was supported, in part, by a University of Alberta Doctoral Recruitment Scholarship. Author contributions: Conceptualization: P.G., C.V., E.S.; Methodology: P.G., C.V., T.Z., E.S.; Validation: P.G., C.V., T.Z., E.S.; Formal analysis: P.G., C.V., T.Z., E.S.; Investigation: P.G., C.V., T.Z., E.S.; Writing - original draft: P.G., C.V., E.S.; Writing - review & editing: P.G., C.V., T.Z., E.S.; Visualization: P.G., C.V., T.Z., E.S.; Supervision: E.S.; Project administration: E.S.; Funding acquisition: E.S. The authors declare no competing or financial interests.

Attached Files

Published - dev187666.full.pdf

Submitted - Vein_Patterning_by_Tissue-Specific_Auxin_Transport.pdf

Supplemental Material - DEV187666supp.pdf

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Additional details

Created:
August 19, 2023
Modified:
October 20, 2023