Published July 1, 2008
| Accepted Version + Supplemental Material
Journal Article
Open
A Shotgun Proteomic Method for the Identification of Membrane-Embedded Proteins and Peptides
Abstract
Integral membrane proteins perform crucial cellular functions and are the targets for the majority of pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains makes them difficult to work with. Here, we describe a shotgun proteomic method for the high-throughput analysis of the membrane-embedded transmembrane domains of integral membrane proteins which extends the depth of coverage of the membrane proteome.
Additional Information
© 2008 American Chemical Society. Received November 30, 2007; Publication Date:June 7, 2008. The authors thank Jessica Krank and Dr. Robert Murphy for expert assistance in the MS analysis of lipids and Dr. Michael MacCoss for critical reading of the manuscript. Financial support for this work was provided by National Institutes of Health Grants K22-AI059076, R01-AA016171, and R21-DA021744 (C.C.W.) and F31-DA022825 (A.R.B.).Attached Files
Accepted Version - nihms135295.pdf
Supplemental Material - pr700795f-file001.qt
Supplemental Material - pr700795f-file002.qt
Supplemental Material - pr700795f-file005.pdf
Supplemental Material - pr700795f-file006.xls
Supplemental Material - pr700795f-file007.xls
Supplemental Material - pr700795f-file008.pdf
Files
nihms135295.pdf
Files
(139.1 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:f365c8c4b656644a2d3ef59620752da3
|
816.1 kB | Download |
|
md5:5851a57ea4fef2e41a62b914b80a90df
|
2.9 MB | Download |
|
md5:ab97a6645b565f501efc8687ce55e51c
|
1.3 MB | Preview Download |
|
md5:668f7cb3d604f51d415c1a307f544c9f
|
32.3 kB | Preview Download |
|
md5:7bf419ecb86a7ccbb926bdddb10ecfac
|
20.4 kB | Preview Download |
|
md5:e6e4785a7ecb64f025d2118e96d687f4
|
60.2 MB | Download |
|
md5:073b7f49f6bbc6086d216ef2d1db34ba
|
73.7 MB | Download |
Additional details
- PMCID
- PMC2765231
- Eprint ID
- 99680
- DOI
- 10.1021/pr700795f
- Resolver ID
- CaltechAUTHORS:20191105-151019589
- K22-AI059076
- NIH
- R01-AA016171
- NIH
- R21-DA021744
- NIH
- F31-DA022825
- NIH
- Created
-
2019-11-05Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field