Pharmacological characterization of presynaptic calcium currents underlying glutamatergic transmission in the avian auditory brainstem
Abstract
We used whole-cell patch-clamp techniques on presynaptic terminals and postsynaptic neurons of the glutamatergic magnocellularis synapse in the chick auditory brainstem to study the effects of N, P, and L-type calcium channel blockers directly on presynaptic calcium currents and transmitter release. Presynaptic calcium currents and transmitter release were unaffected by nifedipine, blocked partially by omega- agatoxin IVA and completely by omega-conotoxin GVIA. The presynaptic calcium current is a low voltage-activated, noninactivating current and its block by omega-agatoxin IVA was not reversed by repeated depolarization of the presynaptic terminal. Thus, the presynaptic calcium current that underlies transmitter release at the chick magnocellularis synapse differs in some respects from N and P-type calcium currents described in vertebrate neuronal cell bodies.
Additional Information
© 1995 Society for Neuroscience. Received Mar. 15, 1995; revised May 25, 1995; accepted May 26, 1995. This work was supported by NIMH and Searle and McKnight Scholars awards to G.L. We thank M. Konishi, L. O. Trussell, and S. Zhang for valuable discussions during this work and M. S. Brodwick, H. Lester, E. M. Schuman, and two anonymous reviewers for critical reading of this manuscript.Attached Files
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Additional details
- PMCID
- PMC6577991
- Eprint ID
- 99613
- Resolver ID
- CaltechAUTHORS:20191101-093417244
- National Institute of Mental Health (NIMH)
- Searle Scholars Program
- McKnight Foundation
- NIH
- Created
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2019-11-01Created from EPrint's datestamp field
- Updated
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2022-02-17Created from EPrint's last_modified field