A sex difference in the composition of the rodent postsynaptic density
Abstract
SynGAP is a postsynaptic density (PSD) protein that binds to PDZ domains of the scaffold protein PSD-95. We previously reported that heterozygous deletion of synGAP in mice is correlated with increased steady-state levels of other key PSD proteins that bind PSD-95, although the level of PSD-95 remains constant (Walkup et al., 2016). For example, the ratio to PSD-95 of Transmembrane AMPA-Receptor-associated Proteins (TARPs), which mediate binding of AMPA-type glutamate receptors to PSD-95, was increased in young synGAP+/- mice. Here we show that a highly significant increase in TARP in the PSDs of young synGAP+/- rodents is present only in females and not in males. The data reveal a sex difference in the adaptation of the PSD scaffold to synGAP heterozygosity.
Additional Information
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. bioRxiv preprint first posted online Oct. 12, 2019. This work was supported by U. S. National Institute of Mental Health Grant MH115456 to MBK, the Allen and Lenabelle Davis Foundation (MBK), U.S. National Science Foundation Fellowship 1612289 to TLM, the Department of Biotechnology, India (SC and PCK), Simons Foundation Autism Research Initiative Grant 529085 to PK, Patrick Wild Centre (SMT and PCK), and Medical Reasearch Council UK Grant MR/P006213/1 to SMT and PCK.Attached Files
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Additional details
- Eprint ID
- 99255
- Resolver ID
- CaltechAUTHORS:20191014-134400805
- NIH
- MH115456
- Allen and Lenabelle Davis Foundation
- NSF
- 1612289
- Department of Biotechnology (India)
- Simons Foundation
- 529085
- Patrick Wild Centre
- Medical Research Council (UK)
- MR/P006213/1
- Created
-
2019-10-14Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field