A rhlI 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in Pseudomonas aeruginosa
Abstract
N-Acyl homoserine lactone (AHL) quorum sensing (QS) controls expression of over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the LasR-LasI circuit and the RhlR-RhlI system. We mapped transcription termination sites affected by AHL QS in P. aeruginosa, and in doing so we identified AHL-regulated small RNAs (sRNAs). Of interest, we noted that one particular sRNA was located within the rhlI locus. We found that rhlI, which encodes the enzyme that produces the AHL N-butanoyl-homoserine lactone (C4-HSL), is controlled by a 5′ untranslated region (UTR)-derived sRNA we name RhlS. We also identified an antisense RNA encoded opposite the beginning of the rhlI open reading frame, which we name asRhlS. RhlS accumulates as wild-type cells enter stationary phase and is required for the production of normal levels of C4-HSL through activation of rhlI translation. RhlS also directly posttranscriptionally regulates at least one other unlinked gene, fpvA. The asRhlS appears to be expressed at maximal levels during logarithmic growth, and we suggest RhlS may act antagonistically to the asRhlS to regulate rhlI translation. The rhlI-encoded sRNAs represent a novel aspect of RNA-mediated tuning of P. aeruginosa QS.
Additional Information
© 2019 Thomason et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received August 31, 2019. Accepted September 11, 2019. Published online October 8, 2019. We thank M. Schuster, A. Dandakar, P. Mandin, E. Fozo, and H. Schweizer for providing strains and plasmids. This work was supported by U.S. Public Health Service (USPHS) grant GM59026 to E.P.G. and European Research Council (ERC) grant ERC-CoG 681203 to R.S. Support for M.K.T. was provided by a Cystic Fibrosis Foundation Postdoctoral Fellowship. M.V. is a Clore Scholar and was supported by the Clore Israel Foundation.Attached Files
Published - mBio-2019-Thomason-e02253-19.full.pdf
Supplemental Material - inline-supplementary-material-1.pdf
Supplemental Material - inline-supplementary-material-2.xlsx
Supplemental Material - inline-supplementary-material-3.pdf
Supplemental Material - inline-supplementary-material-4.pdf
Supplemental Material - inline-supplementary-material-5.pdf
Supplemental Material - inline-supplementary-material-6.pdf
Supplemental Material - inline-supplementary-material-7.pdf
Supplemental Material - inline-supplementary-material-8.pdf
Supplemental Material - inline-supplementary-material-9.pdf
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Additional details
- PMCID
- PMC6786874
- Eprint ID
- 99134
- Resolver ID
- CaltechAUTHORS:20191008-075652504
- GM59026
- NIH
- 681203
- European Research Council (ERC)
- Cystic Fibrosis Foundation
- Clore Israel Foundation
- Created
-
2019-10-08Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field