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Published September 2019 | Published + Supplemental Material
Journal Article Open

A rhlI 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in Pseudomonas aeruginosa

Abstract

N-Acyl homoserine lactone (AHL) quorum sensing (QS) controls expression of over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the LasR-LasI circuit and the RhlR-RhlI system. We mapped transcription termination sites affected by AHL QS in P. aeruginosa, and in doing so we identified AHL-regulated small RNAs (sRNAs). Of interest, we noted that one particular sRNA was located within the rhlI locus. We found that rhlI, which encodes the enzyme that produces the AHL N-butanoyl-homoserine lactone (C4-HSL), is controlled by a 5′ untranslated region (UTR)-derived sRNA we name RhlS. We also identified an antisense RNA encoded opposite the beginning of the rhlI open reading frame, which we name asRhlS. RhlS accumulates as wild-type cells enter stationary phase and is required for the production of normal levels of C4-HSL through activation of rhlI translation. RhlS also directly posttranscriptionally regulates at least one other unlinked gene, fpvA. The asRhlS appears to be expressed at maximal levels during logarithmic growth, and we suggest RhlS may act antagonistically to the asRhlS to regulate rhlI translation. The rhlI-encoded sRNAs represent a novel aspect of RNA-mediated tuning of P. aeruginosa QS.

Additional Information

© 2019 Thomason et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received August 31, 2019. Accepted September 11, 2019. Published online October 8, 2019. We thank M. Schuster, A. Dandakar, P. Mandin, E. Fozo, and H. Schweizer for providing strains and plasmids. This work was supported by U.S. Public Health Service (USPHS) grant GM59026 to E.P.G. and European Research Council (ERC) grant ERC-CoG 681203 to R.S. Support for M.K.T. was provided by a Cystic Fibrosis Foundation Postdoctoral Fellowship. M.V. is a Clore Scholar and was supported by the Clore Israel Foundation.

Attached Files

Published - mBio-2019-Thomason-e02253-19.full.pdf

Supplemental Material - inline-supplementary-material-1.pdf

Supplemental Material - inline-supplementary-material-2.xlsx

Supplemental Material - inline-supplementary-material-3.pdf

Supplemental Material - inline-supplementary-material-4.pdf

Supplemental Material - inline-supplementary-material-5.pdf

Supplemental Material - inline-supplementary-material-6.pdf

Supplemental Material - inline-supplementary-material-7.pdf

Supplemental Material - inline-supplementary-material-8.pdf

Supplemental Material - inline-supplementary-material-9.pdf

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Additional details

Created:
August 19, 2023
Modified:
October 18, 2023