Genome Elimination Mediated by Gene Expression from a Selfish Chromosome
Abstract
Numerous plants and animals harbor selfish B chromosomes that "drive" or transmit themselves at super-Mendelian frequencies, despite long-term fitness costs to the organism. Currently, it is unknown how B chromosome drive is mediated, and whether B-gene expression plays a role. We used modern sequencing technologies to analyze the fine-scale sequence composition and expression of paternal sex ratio (PSR), a B chromosome in the jewel wasp Nasonia vitripennis. PSR causes female-to-male conversion by destroying the sperm's hereditary material in young embryos to drive. Using RNA interference, we demonstrate that testis-specific expression of a PSR-linked gene, named haploidizer, facilitates this genome elimination–and–sex conversion effect. haploidizer encodes a putative protein with a DNA binding domain, suggesting a functional link with the sperm-derived chromatin.
Additional Information
© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). Submitted 24 October 2019; Accepted 14 January 2020; Published 3 April 2020. We thank V. Kumar for help with genomic library preparations. Sequencing was performed at the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology. This work was supported in part by funding from generous UCSD laboratory startup funds awarded to O.S.A. and NSF funding awarded to P.M.F. (NSF-1451839). Author contributions: O.S.A. and P.M.F. conceptualized the study. E.D.B., I.A., T.Y., P.M.F., and H.Q.M.N. performed the various genetic, cytological, and molecular experiments and the bioinformatic analyses in the study. All authors contributed to the writing, analyzed the data, and approved the final manuscript. Competing interests: The authors declare that they have no significant competing financial, professional, or personal interests that might have influenced the performance or presentation of the work described. Data and materials availability: All Illumina, PacBio, and Oxford Nanopore sequencing data can be found at NCBI under accession number PRJNA575073 (https://dataview.ncbi.nlm.nih.gov/object/PRJNA575073?reviewer=uslhbl0iop93gab77lb31ts0n). Previously published RNA-seq data (18) were also used in this study. They can be found under the accession number PRJNA212516 (www.ncbi.nlm.nih.gov/bioproject/PRJNA212516). All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. Additional data related to this paper may be requested from the authors.Attached Files
Published - aaz9808.pdf
Submitted - 793273.full.pdf
Supplemental Material - aaz9808_Data_S1.gz
Supplemental Material - aaz9808_Data_S2.zip
Supplemental Material - aaz9808_Data_S3.zip
Supplemental Material - aaz9808_Data_S4.zip
Supplemental Material - aaz9808_Data_S5.txt
Supplemental Material - aaz9808_Data_S6.zip
Supplemental Material - aaz9808_SM.pdf
Supplemental Material - aaz9808_Tables_S1_to_S10.xlsx
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Additional details
- PMCID
- PMC7124933
- Eprint ID
- 99117
- Resolver ID
- CaltechAUTHORS:20191007-133644493
- University of California, San Diego
- NSF
- MCB-1451839
- Created
-
2019-10-07Created from EPrint's datestamp field
- Updated
-
2022-02-15Created from EPrint's last_modified field
- Caltech groups
- Millard and Muriel Jacobs Genetics and Genomics Laboratory