Published 2009
| public
Book Section - Chapter
In Vivo Studies of Receptors and Ion Channels with Unnatural Amino Acids
- Creators
-
Dougherty, D. A.
Abstract
The combination of nonsense suppression for unnatural amino acid incorporation and heterologous expression in Xenopus oocytes provides a powerful means to probe neuroreceptors and ion channels with chemical precision. Here we describe a range of studies that illustrate the broad potential of this approach. A variety of biophysical probes and reactive moieties can be incorporated. In addition, subtle systematic variations allow detailed, physical organic chemistry studies of the complex proteins of neuroscience.
Additional Information
© 2009 Springer-Verlag Berlin Heidelberg. Our work on unnatural amino acid mutagenesis is part of an enjoyable and productive collaboration with Henry Lester, Division of Biology, California Institute of Technology. We have also benefited from fruitful collaborations with Sarah Lummis, Cambridge University, and Richard Horn, Jefferson Medical College. I am very grateful to the many exceptional students who have done all the work and provided many of the creative insights described here. Our work has been supported by the NIH (NS 34407).Additional details
- Eprint ID
- 98534
- Resolver ID
- CaltechAUTHORS:20190909-161656237
- NS 34407
- NIH
- Created
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2019-09-09Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field
- Series Name
- Nucleic Acids and Molecular Biology
- Series Volume or Issue Number
- 22