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Published June 4, 2019 | Published + Accepted Version + Supplemental Material
Journal Article Open

Müller Glial Cells Participate in Retinal Waves via Glutamate Transporters and AMPA Receptors

Abstract

Retinal waves, the spontaneous patterned neural activities propagating among developing retinal ganglion cells (RGCs), instruct the activity-dependent refinement of visuotopic maps. Although it is known that the wave is initiated successively by amacrine cells and bipolar cells, the behavior and function of glia in retinal waves remain unclear. Using multiple in vivo methods in larval zebrafish, we found that Müller glial cells (MGCs) display wave-like spontaneous activities, which start at MGC processes within the inner plexiform layer, vertically spread to their somata and endfeet, and horizontally propagate into neighboring MGCs. MGC waves depend on glutamatergic signaling derived from bipolar cells. Moreover, MGCs express both glia-specific glutamate transporters and the AMPA subtype of glutamate receptors. The AMPA receptors mediate MGC calcium activities during retinal waves, whereas the glutamate transporters modulate the occurrence of retinal waves. Thus, MGCs can sense and regulate retinal waves via AMPA receptors and glutamate transporters, respectively.

Additional Information

© 2019 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Received 23 July 2018, Revised 21 February 2019, Accepted 30 April 2019, Available online 4 June 2019. We are grateful to Dr. Pamela A. Raymond for providing the Tg(GFAP:eGFP) line and to Dr. Xiaoquan Li for making the Tg(GFAP:GCaMP2) line. This work was supported by the National Natural Science Foundation of China (31771144), the Key Research Program of Frontier Sciences (QYZDY-SSW-SMC028), the Strategic Priority Research Program (XDB32010200), the Youth Innovation Promotion Association of Chinese Academy of Sciences, Shanghai Municipal Science and Technology Major Project (18JC1410100 and 2018SHZDZX05), the China Wan-Ren Program, and the Shanghai Leading Scientist Program. The ORCID for R.-W.Z. is 0000-0003-1145-8589. Author Contributions: R.-W.Z. and J.-L.D. conceived the project and designed the experiments. R.-W.Z. and W.-J.D. performed the research and analyzed the data. D.A.P. provided some experimental reagents. R.-W.Z. and J.-L.D. wrote the paper. The authors declare no competing interests.

Attached Files

Published - 1-s2.0-S2211124719306229-main.pdf

Accepted Version - nihms-1534459.pdf

Supplemental Material - 1-s2.0-S2211124719306229-mmc1.pdf

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Additional details

Created:
August 22, 2023
Modified:
October 20, 2023