Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published May 6, 2009 | Published
Book Section - Chapter Open

Harmonic holography for three-dimensional cellular imaging

Abstract

Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO_3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and a broad flexibility in the choice of excitation wavelength. We use the coherent SHG signal from BaTiO_3 nanoparticles for three-dimensional (3D) imaging without scanning. We built a harmonic holographic (H^2) microscope which records digital holograms at the second harmonic frequency. Highresolution 3D distributions of these SHG markers in mammalian cells are successfully captured and interpreted by the H^2 microscope.

Additional Information

© 2009 Society of Photo-Optical Instrumentation Engineers (SPIE).

Attached Files

Published - 73290H.pdf

Files

73290H.pdf
Files (1.8 MB)
Name Size Download all
md5:ebf532042630a8f38137df7f4285ef68
1.8 MB Preview Download

Additional details

Created:
August 20, 2023
Modified:
January 14, 2024