Harmonic holography for three-dimensional cellular imaging
Abstract
Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO_3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and a broad flexibility in the choice of excitation wavelength. We use the coherent SHG signal from BaTiO_3 nanoparticles for three-dimensional (3D) imaging without scanning. We built a harmonic holographic (H^2) microscope which records digital holograms at the second harmonic frequency. Highresolution 3D distributions of these SHG markers in mammalian cells are successfully captured and interpreted by the H^2 microscope.
Additional Information
© 2009 Society of Photo-Optical Instrumentation Engineers (SPIE).Attached Files
Published - 73290H.pdf
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Additional details
- Eprint ID
- 95542
- Resolver ID
- CaltechAUTHORS:20190516-111740618
- Created
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2019-05-16Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field
- Series Name
- Proceedings of SPIE