Genetic dissection of developmental pathways
- Creators
- Huang, Linda S.
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Sternberg, Paul W.
Abstract
A major tool of developmental geneticists is the ordering of genes in functional pathways. In this chapter, we explain the logic behind constructing pathways, starting from the knowledge of the relevant phenotypes associated with the genes of interest, assuming that careful analysis of the phenotype has been carried out. We discuss the construction and interpretation of phenotypes of double mutants, screening for and analysis of extragenic suppressors, as well as issues regarding complex pathways and genetic redundancy. Avery and Wasserman (1992) have provided a brief theoretical discussion of epistasis analysis; here we explain the more practical aspects of how models of developmental pathways are built in C. elegans.
Additional Information
© 2006 WormBase. All WormBook content, except where otherwise noted, is licensed under a Creative Commons Attribution License. Edited by David Fay. WormMethods editor, Victor Ambros. Last revised April 22, 2005. Published June 14, 2006. The authors would like to thank Paul Garrity, Wendy Katz, Ralf Sommer, John DeModena, Marie-Anne Félix, Yvonne Hadju-Cronin, Howard Lipshitz, Katharine Liu, Anna Newman, and the editors for critical comments. Research in our laboratory discussed here was supported by the U. S .P. H. S, the March of Dimes Birth Defects Foundation, and the Howard Hughes Medical Institute, with which P. W. S. is an Investigator.Attached Files
Published - epistasis.pdf
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Additional details
- Eprint ID
- 95458
- Resolver ID
- CaltechAUTHORS:20190513-161611318
- NIH
- March of Dimes Birth Defects Foundation
- Howard Hughes Medical Institute (HHMI)
- Created
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2019-05-14Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field