Molecular Imaging Cellular SPIO Uptake with Nonlinear Optical Microscopy
Abstract
We report a novel application to demonstrate and visualize the selective binding of lipids in cells of the reticuloendothelial system to super paramagnetic iron oxide(SPIO) nanoparticles. Ten New Zealand White rabbits that were experimentally injected intravenously with SPIO and five controls were investigated with vibrational microspectroscopy based on surface-enhanced coherent anti-Stokes Raman scattering (SECARS) microscopy. Marked cellular intensity enhancements in hepatic Kupffer cells and melanomacrophages of spleen have been observed in the range of 2850–2875 cm^(−1) in SPIO-injected animals but not in controls. The enhancements are related to the selective association of lipid molecules in cells of the reticuloendothelial system to uptaken SPIO, which can uniquely be visualized with SECARS microscopy.
Additional Information
© 2010 Elsevier Inc. Available online 1 October 2010. This project was supported by a grant from the Medizinische Forschungsförderung Innsbruck MFI (MCW, Project 9443).Additional details
- Eprint ID
- 95421
- Resolver ID
- CaltechAUTHORS:20190513-083521741
- 9443
- Medizinische Forschungsförderung Innsbruck
- Created
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2019-05-13Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field