Isolation and Partial Characterization of a Mutant of Escherichia coli Deficient in DNA Polymerase II
Abstract
A mutant of Escherichia coli deficient in DNA polymerase II has been isolated from E. coli polA1 by mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine and assay of polymerase activity in extracts of survivors. The polA1 mutation was suppressed during mutagenesis by introduction of the suppressor, su7^+, into the parental strain. The mutant, HMS83 polA1 polB1, contains less than 0.5% of the normal levels of DNA polymerase II. The only polymerase activity detected in the mutant is DNA polymerase III. E. coli HMS83 grows normally at both 25 and 42°, and supports the growth of bacteriophages T4, T7, lambda, ϕX174, 186, P2, and fd. The polB mutation does not affect sensitivity to ultraviolet irradiation or recombination frequencies.
Additional Information
© 1972 National Academy of Sciences. Communicated by Eugene P. Kennedy, May 26, 1972. We thank Henry Mulcahy and Robert DiNello for their excellent assistance, and Dr. Hiroaki Shizuya for his helpful advice. This investigation was supported by research grants from the National Institutes of Health, United States Public Health Service Grant AI-06045, and Grant P486 from the American Cancer Society, Inc. C. C. R. is the recipient of a Public Health Service Research Career Program Award, no. GM-13,534.Attached Files
Published - pnas00134-0100.pdf
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Additional details
- PMCID
- PMC426875
- Eprint ID
- 95396
- Resolver ID
- CaltechAUTHORS:20190510-105529866
- NIH
- AI-06045
- American Cancer Society
- P486
- NIH
- GM-13534
- Created
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2019-05-10Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field