Published August 1974
| Published
Journal Article
Open
Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12
Abstract
Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr x F^- crosses, F-ductants in episome transfer, and transductants in generalized transduction by phage P1. The polB gene is located at 2 min on the Escherichia coli linkage map; it is 39 to 64% co-transducible with leu. A mutant, E. coli polA1 polB100 polC (ts), deficient in deoxyribonucleic acid polymerases I and II and having a thermolabile deoxyribonucleic acid polymerase III, has been prepared by the P1-mediated cross: P1 (HMS85 polB100) x E. coli BT1026 polA1 polC (ts) leu^-.
Additional Information
© 1974 American Society for Microbiology. Received for publication 26 April 1974. This investigation was supported by Public Health Service grant AI-06045 from the National Institute of Allergy and Infectious Diseases, grant NP-1C from the American Cancer Society, Incorporated, and Public Health Service Research Career Development Award GM 13,634 (to C.C.R.) from the National Institute of General Medical Sciences.Attached Files
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Additional details
- PMCID
- PMC245632
- Eprint ID
- 95389
- Resolver ID
- CaltechAUTHORS:20190510-105514765
- AI-06045
- NIH
- NP-1C
- American Cancer Society
- GM 13634
- NIH
- Created
-
2019-05-10Created from EPrint's datestamp field
- Updated
-
2019-10-03Created from EPrint's last_modified field