Transcription of cis-antisense small RNA MtlS in Vibrio cholerae is regulated by transcription of its target gene mtlA
- Creators
- Zhang, Mark G.
- Liu, Jane M.
Abstract
Vibrio cholerae, the facultative pathogen responsible for cholera disease, continues to pose a global health burden. Its persistence can be attributed to a flexible genetic tool kit that allows for adaptation to different environments with distinct carbon sources, including the six-carbon sugar alcohol mannitol. V. cholerae takes up mannitol through the transporter protein MtlA, whose production is downregulated at the posttranscriptional level by MtlS, a cis antisense small RNA (sRNA) whose promoter lies within the mtlA open reading frame. Though it is known that mtlS expression is robust under growth conditions lacking mannitol, it has remained elusive as to what factors govern the steady-state levels of MtlS. Here, we show that manipulating mtlA transcription is sufficient to drive inverse changes in MtlS levels, likely through transcriptional interference. This work has uncovered a cis-acting sRNA whose expression pattern is predominantly controlled by transcription of the sRNA's target gene.
Additional Information
© 2019 Zhang and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received 7 March 2019; Accepted 22 April 2019; Accepted manuscript posted online 29 April 2019; Published 21 June 2019. M.G.Z. and J.M.L. conceived of and performed all of the experiments. M.G.Z. and J.M.L. wrote the paper. J.M.L. supervised the study. We thank Len Seligman, Pete Chandrangsu, and members of the J. M. Liu lab for their feedback and discussions during preparation of the manuscript. The research was funded by an NIH grant to J.M.L. (AI090606), the Arnold and Mabel Beckman Foundation, the Camille and Henry Dreyfus Foundation, and Pomona College.Attached Files
Published - Journal_of_Bacteriology-2019-Zhang-e00178-19.full.pdf
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Additional details
- Alternative title
- mtlA transcription regulates cis-antisense sRNA MtlS
- PMCID
- PMC6597380
- Eprint ID
- 95079
- Resolver ID
- CaltechAUTHORS:20190429-143530710
- AI090606
- NIH
- Arnold and Mabel Beckman Foundation
- Camille and Henry Dreyfus Foundation
- Pomona College
- Created
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2019-04-29Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field