Following cell fate in the living mouse embryo
Abstract
It has been difficult to follow many of the dramatic changes in cell fate and cell migration during mouse development. This is because there has been no enduring marker that would allow cells to be recognised in the living embryo. We believe that we have overcome this problem by developing a novel form of green fluorescent protein, named MmGFP, that proves to be easily visible and non toxic to mouse cells and does not perturb embryogenesis. We show that synthetic mRNA encoding MmGFP can be injected into blastomeres to follow the fate of their progeny during preimplantation development. We have made a stable embryonic stem cell line that expresses MmGFP and introduced these fluorescent cells into mouse embryos. For the first time, we have been able to follow the fate of embryonic stem cells in living embryos and to observe directly the contribution of these cells to distinct lineages of the postimplantation embryo. This approach should lead to a more complete description of the dynamics of cell fate in the mouse.
Additional Information
© 1997 The Company of Biologists. (Accepted 20 January 1997) We thank Brendan Cormack for communicating results prior to publication, Hartmund Land for the cdc2 promoter and numerous colleagues for helpful discussions. M. Z.-G. thanks the EMBO for a long-term postdoctoral fellowship. This work was supported by funds from the Wellcome Trust to M. J. E., the MRC to J. H. and the Cancer Research Campaign to J. P.Attached Files
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Additional details
- Eprint ID
- 94846
- Resolver ID
- CaltechAUTHORS:20190422-101710597
- European Molecular Biology Organization (EMBO)
- Wellcome Trust
- Cancer Research Campaign
- Created
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2019-04-23Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field