Efficient delivery of dsRNA into zona-enclosed mouse oocytes and preimplantation embryos by electroporation
Abstract
Conditions for the electroporation of mouse oocytes and preimplantation embryos have been optimised by following the incorporation of rhodamine labeled dextran. This procedure includes a step to weaken but not remove the zona pellucida that helps achieve good survival. This approach has been applied to introduce double‐stranded RNA for c‐mos into oocytes and green fluorescent protein (GFP) into transgenic GFP‐expressing embryos at the 1‐ and 4‐cell stages. In both cases we were able to observe sequence‐specific interference with the expression of the target gene—a failure of oocytes to arrest at metaphase II and a loss in the green fluorescence of embryos by the morula or blastocyst stages. These effects could be observed in multiple oocytes or embryos allowed to develop together following electroporation.
Additional Information
© 2002 Wiley‐Liss. Received 12 November 2001; Accepted 18 January 2002. We are grateful to CRC Technology for their support of this work. MZG is a fellow of the Lister Institute of Preventive Medicine.Additional details
- Eprint ID
- 94839
- Resolver ID
- CaltechAUTHORS:20190422-100408907
- Cancer Research Campaign Technology
- Lister Institute of Preventive Medicine
- Created
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2019-04-23Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field