Developmental fate of embryonic germ cells (EGCs), in vivo and in vitro
Abstract
Embryonic germ cells (EGCs) derived from mouse primordial germ cells (PGCs) are known both to colonize all cell lineages of the fetus and to make tumors in vivo. When aggregated with eight-cell embryos, EGCs from a new EGC line expressing green fluorescent protein (GFP) were found to contribute preferentially to the epiblast but unexpectedly were also capable of colonizing primary endoderm. When injected under the kidney capsule, EGCs derived from 12.5 days post coitum (dpc) PGCs formed differentiated tumors. The ability of EGCs to differentiate in an organ culture system depends upon their partners in cell culture. When EGCs, marked with a LacZ transgene, were mixed with disaggregated and reaggregated mouse fetal lung in an organ culture system, they remained undifferentiated. In urogenital ridge reaggregates on the other hand, some EGCs were capable of differentiating to form small epithelial cysts.
Additional Information
© 2003 International Society of Differentiation. Published by Elsevier. Accepted 21 October 2002, Available online 23 November 2009. We are grateful to the Wellcome Trust for financial support (GD-H, MZG, and AM). We are also grateful for financial support to the Cancer Research Campaign (MZG and FW) and the Lister Institute of Preventive Medicine (MZG). We thank Dr. Sheila Barton for her help in both injecting the construct to produce EF1alpha MmGFP transgenic mice and injection of EGCs under the kidney capsule and Dr Takeshi Tada for allowing us to use his LacZ-expressing EGCs.Additional details
- Eprint ID
- 94828
- Resolver ID
- CaltechAUTHORS:20190419-105935323
- Wellcome Trust
- Cancer Research Campaign
- Lister Institute of Preventive Medicine
- Created
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2019-04-23Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field