Concerted cell divisions in embryonic visceral endoderm guide anterior visceral endoderm migration
Abstract
Migration of Anterior Visceral Endoderm (AVE) is a critical symmetry breaking event in the early post-implantation embryo development and is essential for establishing the correct body plan. Despite much effort, cellular and molecular events influencing AVE migration are only partially understood. Here, using time-lapse live imaging of mouse embryos, we demonstrate that cell division in the embryonic visceral endoderm is coordinated with AVE migration. Moreover, we demonstrate that temporal inhibition of FGF signalling during the pre-implantation specification of embryonic visceral endoderm perturbs cell cycle progression, thus affecting AVE migration. These findings demonstrate that coordinated cell cycle progression during the implantation stages of development is important for post-implantation morphogenesis in the mouse embryo.
Additional Information
© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received 3 December 2018, Revised 26 March 2019, Accepted 26 March 2019, Available online 30 March 2019. The authors declare no competing or financial interests. Author contribution: F.A. designed, performed and analysed all the experiments. L.C.O. helped with some experiments and analysis of data. R.L.M. provided the R26Fucci2a mouse line. F.A. and M.Z.G. conceived the project and wrote the manuscript. This work was supported by Wellcome Trust (098287/Z/12/Z) and European Research Council (669198) grants to M.Z.G., F.A. was supported by an EMBO postdoctoral fellowship and L.C.O. was supported by a Centre for Trophoblast Research PhD studentship. We thank D. Glover, M. N. Shahbazi, N. Christodoulou, C. Kyprianou and A. Cox for discussion and feedback and W. Mansfield for help with embryo transfer.Attached Files
Published - 1-s2.0-S0012160618307954-main.pdf
Supplemental Material - 1-s2.0-S0012160618307954-figs1_lrg.jpg
Supplemental Material - 1-s2.0-S0012160618307954-figs2_lrg.jpg
Supplemental Material - 1-s2.0-S0012160618307954-figs3_lrg.jpg
Supplemental Material - 1-s2.0-S0012160618307954-figs4_lrg.jpg
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Additional details
- PMCID
- PMC6553843
- Eprint ID
- 94562
- Resolver ID
- CaltechAUTHORS:20190408-111045626
- 098287/Z/12/Z
- Wellcome Trust
- 669198
- European Research Council (ERC)
- European Molecular Biology Organization (EMBO)
- Centre for Trophoblast Research
- Created
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2019-04-09Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field