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Published February 21, 2013 | Supplemental Material + Published
Journal Article Open

Asymmetric Localization of Cdx2 mRNA during the First Cell-Fate Decision in Early Mouse Development

Abstract

A longstanding question in mammalian development is whether the divisions that segregate pluripotent progenitor cells for the future embryo from cells that differentiate into extraembryonic structures are asymmetric in cell-fate instructions. The transcription factor Cdx2 plays a key role in the first cell-fate decision. Here, using live-embryo imaging, we show that localization of Cdx2 transcripts becomes asymmetric during development, preceding cell lineage segregation. Cdx2 transcripts preferentially localize apically at the late eight-cell stage and become inherited asymmetrically during divisions that set apart pluripotent and differentiating cells. Asymmetric localization depends on a cis element within the coding region of Cdx2 and requires cell polarization as well as intact microtubule and actin cytoskeletons. Failure to enrich Cdx2 transcripts apically results in a significant decrease in the number of pluripotent cells. We discuss how the asymmetric localization and segregation of Cdx2 transcripts could contribute to multiple mechanisms that establish different cell fates in the mouse embryo.

Additional Information

© 2013 The Authors. Published by Elsevier Under a Creative Commons license (Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)) Received 4 February 2012, Revised 1 June 2012, Accepted 7 January 2013, Available online 31 January 2013. We are grateful to Simon Bullock, David Glover, Claire Chazaud, Rui Pires Martins, Alexander Bruce, Kitai Kim, Isabel Palacios, and Alejandra Gardiol for help and advice. This work was supported by a Wellcome Trust Senior Research Fellowship to M.Z.G. M.S. was funded by the Greek State Scholarships Foundation.

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Published - 1-s2.0-S2211124713000132-main.pdf

Supplemental Material - 1-s2.0-S2211124713000132-mmc1.mov

Supplemental Material - 1-s2.0-S2211124713000132-mmc2.mov

Supplemental Material - 1-s2.0-S2211124713000132-mmc3.mov

Supplemental Material - 1-s2.0-S2211124713000132-mmc4.mov

Supplemental Material - 1-s2.0-S2211124713000132-mmc5.mov

Supplemental Material - 1-s2.0-S2211124713000132-mmc6.mov

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Additional details

Created:
August 19, 2023
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