Published April 2019 | public
Conference Paper

Enzymatic labeling of bacterial proteins for live-cell super-resolution imaging

An error occurred while generating the citation.

Abstract

Mol. probes that illuminate the subcellular organization of prokaryotic organisms are essential for our understanding of the biol. world. We describe here a strategy for labeling bacterial proteins in situ with azido fatty acids using the enzyme N-myristoyltransferase. Cell-permeant photoswitchable fluorescent dyes are conjugated to target proteins through means of strain-promoted azide-alkyne cycloaddn. to enable super-resoln. imaging in live Escherichia coli cells. We demonstrate the method by labeling and imaging bacterial chemotaxis proteins and cell division proteins. The method provides a general framework for live-cell protein imaging with nanometer resoln.

Additional Information

© 2019 American Chemical Society.

Additional details

Created:
August 19, 2023
Modified:
October 20, 2023