Design and performance of in vitro transcription rate regulatory circuits
- Creators
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Franco, Elisa
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Murray, Richard M.
Abstract
This paper proposes a synthetic in vitro circuit that aims at regulating the rate of RNA transcription through positive feedback interactions. This design is dual to a previously synthesized transcriptional rate regulator based on self-repression. Two DNA templates are designed to interact through their transcripts, creating cross activating feedback loops that will equate their transcription rates at steady state. A mathematical model is developed for this circuit, consisting of a set of ODEs derived from the mass action laws and Michaelis-Menten kinetics involving all the present chemical species. This circuit is then compared to its regulatory counterpart based on negative feedback. A global sensitivity analysis reveals the fundamental features of the two designs by evaluating their equilibrium response to changes in the most crucial parameters of the system.
Additional Information
© 2008 IEEE. Research supported in part by the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the U.S. Army Research Office. The authors would like to thank Erik Winfree, Jongmin Kim, Per-Ola Forsberg and all the members of the DNA and Natural Algorithms group at Caltech for their helpful advise during the development of this project.Attached Files
Published - 04739253.pdf
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Additional details
- Eprint ID
- 94071
- Resolver ID
- CaltechAUTHORS:20190322-143454266
- Army Research Office (ARO)
- DAAD19-03-D-0004
- Created
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2019-03-22Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field