Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells
Abstract
Background: Amoora rohituka is described in Ayurveda, an Indian traditional system of medicine for management of disorders of blood, diseases of eye, helminthiasis disease, ulcer, liver disorders and splenomegaly. However, the leaves were not reported to have anticancer properties till date. Objective: This study was carried out to evaluate the cytotoxic potential of leaf extracts of Amoora rohituka. Materials and methods: The leaves powder was macerated in petroleum ether, ethyl acetate and methanol and evaluated their anticancer activities in vitro. The phytochemical constituents of the active (ethyl acetate) extract were screened by FTIR analysis and phytochemical screening methods. Results: The ethyl acetate extract (RLEA) showed the presence of alkaloids, flavonoids, steroids, tannins, saponins and terpenoids. The RLEA exhibited high cytotoxic effect against human breast cancer cells, MCF-7 (IC50 = 9.81 μg/mL) and induced apoptosis by altering nuclear morphology and DNA laddering. Wound healing assays explained the potency of extract to decrease the cell migration. Conclusion: The extract of Amoora rohituka leaves exhibited anticancer activity with less toxicity and it could be used for development of alternative drugs in the treatment of human breast cancer.
Additional Information
© 2019 The Authors. Published by Elsevier B.V. on behalf of Institute of Transdisciplinary Health Sciences and Technology and World Ayurveda Foundation. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Received 13 August 2018, Revised 8 December 2018, Accepted 22 December 2018, Available online 4 March 2019. We are thankful to the Centre of Experimental Medicine & Surgery (CEMS), Institute of Medical Sciences, Banaras Hindu University, Varanasi, India for instrument facilities. Source(s) of funding: The author Rajesh Kumar Singh is grateful to BHU and UGC for financial supports as fellowships (No. R/Dev/Scholarship (SRF-JRF)/Ayurveda/57032. Conflict of interest: None.Attached Files
Published - 1-s2.0-S0975947618307204-main.pdf
Files
Name | Size | Download all |
---|---|---|
md5:69850d4ff372957afd3e3a9bee18a055
|
2.1 MB | Preview Download |
Additional details
- PMCID
- PMC7772503
- Eprint ID
- 93445
- Resolver ID
- CaltechAUTHORS:20190304-134833117
- Banaras Hindu University
- 57032
- University Grants Commission
- Created
-
2019-03-04Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field