Neuronal development in the drosophila retina: Monoclonal antibodies as molecular probes
Abstract
The compound eye of D. melanogaster is a reiterative pattern of facets, each containing eight photoreceptor cells in a precise arrangement. This pattern is established in the eye imaginal disc during the third larval instar. A wave of morphogenesis sweeps from posterior to anterior across the disc, leaving in its wake organized clusters of photoreceptor cells. We have used monoclonal antibodies to highlight pattern elements that are not readily observable by other techniques. Monoclonal antibodies can be used to identify the molecules associated with particular patterns, providing links between observable structures and the genes. As an example, we present the purification and N-terminal sequence of a glycoprotein antigen specific to photoreceptor cells and their axons.
Additional Information
© 1984 MIT. Received October 18, 1983. We gratefully acknowledge the expert technical assistance of Marika Szalay and Devra Ellert We thank Carol Miller and Mark Tanouye for helpful discussions. The protein sequenator was generously made available by Dr. William Dreyer. This work was supported by National Science Foundation grant PCM 79-11771 (S. B.). fellowships from the Helen Hay Whitney Foundation (S. L. 2.) and the Gosney Foundation (T. R. V.), and National Institutes of Health grant 2-T32-GM07401-06 (D. B. T.). The costs of publication of this article were defrayed rn part by the payment of page charges. Thus article must therefore be hereby marked "advertisement" In accordance with 18 USC. Section 1734 solely to indicate thus fact.Additional details
- Eprint ID
- 93307
- DOI
- 10.1016/0092-8674(84)90069-2
- Resolver ID
- CaltechAUTHORS:20190227-140300257
- PCM 79-11771
- NSF
- Helen Hay Whitney Foundation
- Gosney Foundation
- 2-T32-GM07401-06
- NIH Predoctoral Fellowship
- Created
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2019-02-27Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field