Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans
Abstract
Neurite fasciculation through contact-dependent signaling is important for the wiring and function of the neuronal circuits. Here, we describe a type of axon-dendrite fasciculation in C. elegans, where proximal dendrites of the nociceptor PVD adhere to the axon of the ALA interneuron. This axon-dendrite fasciculation is mediated by a previously uncharacterized adhesive signaling by the ALA membrane signal SAX-7/L1CAM and the PVD receptor SAX-3/Robo but independent of Slit. L1CAM physically interacts with Robo and instructs dendrite adhesion in a Robo-dependent manner. Fasciculation mediated by L1CAM-Robo signaling aligns F-actin dynamics in the dendrite growth cone and facilitates dynamic growth cone behaviors for efficient dendrite guidance. Disruption of PVD dendrite fasciculation impairs nociceptive mechanosensation and rhythmicity in body curvature, suggesting that dendrite fasciculation governs the functions of mechanosensory circuits. Our work elucidates the molecular mechanisms by which adhesive axon-dendrite signaling shapes the construction and function of sensory neuronal circuits.
Additional Information
© 2018 Elsevier Inc. Received 22 May 2018, Revised 18 September 2018, Accepted 26 October 2018, Available online 13 December 2018. We thank Nelson Ramirez and Hannes Bülow for sharing data and suggestions before publication and Paul Sternberg for the slt-1(eh15) strain. The authors are grateful to Hua-Man Hsu for her assistance in confocal microscopy and the First and Third Core Labs, National Taiwan University College of Medicine, for imaging, flow cytometry, and cell sorting. We thank Chih-Chiang Chan for providing the pAc5.1 vector. Some of the strains used in this study are provided by the Caenorhabditis Genetics Center, which is supported by NIH Office of Research Infrastructure Programs (P40OD010440), and by the National BioResources Project (NBRP), Japan. This study was funded by the Ministry of Science and Technology (MOST), Taiwan, to C.-L.P. (MOST 106-2320-B-002-051-MY3) and a postdoctoral fellowship to C.-H.C. (MOST 106-2811-B-002-134). Author Contributions: Conceptualization, C.-H.C. and C.-L.P.; Investigation, C.-H.C., H.-W.H., Y.-H.C., and C.-L.P.; Writing – Original Draft, C.-H.C. and C.-L.P.; Writing – Review & Editing, C.-H.C. and C.-L.P.; Funding Acquisition, C.-L.P.; Supervision, C.-L.P.Attached Files
Supplemental Material - 1-s2.0-S1534580718309171-mmc1.pdf
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Additional details
- Eprint ID
- 92904
- Resolver ID
- CaltechAUTHORS:20190213-152836749
- P40OD010440
- NIH
- National BioResources Project
- MOST 106-2320-B-002-051-MY3
- Ministry of Science and Technology (Taipei)
- MOST 106-2811-B-002-134
- Ministry of Science and Technology (Taipei)
- Created
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2019-02-14Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field