Phototrophic Methane Oxidation in a Member of the Chloroflexi Phylum
Abstract
Biological methane cycling plays an important role in Earth's climate and the global carbon cycle, with biological methane oxidation (methanotrophy) modulating methane release from numerous environments including soils, sediments, and water columns. Methanotrophy is typically coupled to aerobic respiration or anaerobically via the reduction of sulfate, nitrate, or metal oxides, and while the possibility of coupling methane oxidation to phototrophy (photomethanotrophy) has been proposed, no organism has ever been described that is capable of this metabolism. Here we described a new bacterial genome from a member of the Chloroflexi phylum--termed here Candidatus Chlorolinea photomethanotrophicum--with cooccurring methanotrophy and phototrophy pathways, suggesting a novel link between these two metabolisms. Recovered as a metagenome-assembled genome from microbial mats in an iron-rich hot spring in Japan, Ca. "C. photomethanotrophicum" forms a new lineage within the Chloroflexi phylum and expands the known metabolic diversity of this already diverse clade. Ca. "C. photomethanotrophicum" appears to be metabolically versatile, capable of phototrophy (via a Type 2 reaction center), aerobic respiration, nitrite reduction, oxidation of methane and carbon monoxide, and potentially carbon fixation via a novel pathway composed of hybridized components of the serine cycle and the 3-hydroxypropionate bicycle. The biochemical network of this organism is constructed from components from multiple organisms and pathways, further demonstrating the modular nature of metabolic machinery and the ecological and evolutionary importance of horizontal gene transfer in the establishment of novel pathways.
Additional Information
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. LMW acknowledges support from NASA NESSF (#NNX16AP39H), NSF (#OISE 1639454), NSF GROW (#DGE 1144469), the Earth-Life Science Institute Origins Network (EON), and the Agouron Institute. P.M.S. was supported by The Branco Weiss Fellowship - Society in Science from ETH Zurich. WWF acknowledges the generous support of the Caltech Center for Environment Microbe Interactions, NASA Exobiology (#NNX16AJ57G), and the Simons Foundation Collaboration on the Origins of Life (SCOL). SEM is supported by NSF Award 1724300, JSPS KAKENHI Grant Number 18H01325, and the Research Foundation for Opto-Science and Technology.Attached Files
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Additional details
- Eprint ID
- 92525
- DOI
- 10.1101/531582
- Resolver ID
- CaltechAUTHORS:20190130-121936798
- NASA Earth and Space Science Fellowship
- NNX16AP39H
- NSF
- OISE-1639454
- NSF Graduate Research Fellowship
- DGE-1144469
- Earth-Life Science Institute Origins Network (EON)
- Agouron Institute
- ETH Zurich
- Caltech Center for Environmental Microbial Interactions (CEMI)
- NASA
- NNX16AJ57G
- Simons Foundation
- NSF
- EF-1724300
- Japan Society for the Promotion of Science (JSPS)
- 18H01325
- Research Foundation for Opto-Science and Technology
- Created
-
2019-01-31Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field
- Caltech groups
- Caltech Center for Environmental Microbial Interactions (CEMI)