Topological Organization of Ventral Tegmental Area Connectivity Revealed by Viral-Genetic Dissection of Input-Output Relations
Abstract
Viral-genetic tracing techniques have enabled mesoscale mapping of neuronal connectivity by teasing apart inputs to defined neuronal populations in regions with heterogeneous cell types. We previously observed input biases to output-defined ventral tegmental area dopamine (VTA-DA) neurons. Here, we further dissect connectivity in the VTA by defining input-output relations of neurochemically and output-defined neuronal populations. By expanding our analysis to include input patterns to subtypes of excitatory (vGluT2-expressing) or inhibitory (GAD2-expressing) populations, we find that the output site, rather than neurochemical phenotype, correlates with whole-brain inputs of each subpopulation. Lastly, we find that biases in input maps to different VTA neurons can be generated using publicly available whole-brain output mapping datasets. Our comprehensive dataset and detailed spatial analysis suggest that connection specificity in the VTA is largely a function of the spatial location of the cells within the VTA.
Additional Information
© 2018 The Authors. This is an open access article under the CC BY-NC-ND license. Received 1 March 2018, Revised 30 October 2018, Accepted 7 December 2018, Available online 2 January 2019. A special thanks to Takeru Matsuda for assistance with clustering and bootstrapping analysis and Nic Berns for the MATLAB code used to bin axonal projection data. Research was funded by an NIH K99 grant (DA041445) and an NIH F32 grant (DA038913) from NIDA (to K.T.B.) and the Howard Hughes Medical Institute (to L.L.). Author Contributions: K.T.B. designed all experiments and wrote the manuscript with input from R.C.M. and L.L. K.T.B. performed all experiments and collected all the data. X.J.G., S.X., and K.E.D. assisted with data analysis. The authors declare no competing interests.Attached Files
Published - 1-s2.0-S2211124718319703-main.pdf
Accepted Version - nihms-1517822.pdf
Supplemental Material - 1-s2.0-S2211124718319703-mmc1.pdf
Supplemental Material - 1-s2.0-S2211124718319703-mmc2.xlsx
Supplemental Material - 1-s2.0-S2211124718319703-mmc3.xlsx
Supplemental Material - 1-s2.0-S2211124718319703-mmc4.xlsx
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Additional details
- PMCID
- PMC6379204
- Eprint ID
- 92336
- Resolver ID
- CaltechAUTHORS:20190117-085931079
- DA041445
- NIH
- DA038913
- NIH
- Howard Hughes Medical Institute (HHMI)
- Created
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2019-01-22Created from EPrint's datestamp field
- Updated
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2022-02-24Created from EPrint's last_modified field