Defocus-gradient corrected back-projection
- Creators
- Jensen, Grant J.
- Kornberg, Roger D.
Abstract
Three-dimensional reconstructions of icosahedral viruses from cryoelectron microscope images have reached resolutions where the microscope depth of field is a significant resolution-limiting factor. An analytical treatment presented here shows how the depth of field limitation can be understood as an envelope function which gradually attenuates the signal, starting well before the numerical depth of field is actually reached. A simple modification to the well-known back-projection reconstruction algorithm is described, called the defocus-gradient corrected back-projection, which computationally corrects for the contrast transfer function along a defocus gradient. Computer simulations demonstrate how the algorithm effectively eliminates the depth of field limitation.
Additional Information
© 2000 Elsevier. Received 2 September 1999, Revised 20 December 1999, Available online 15 June 2000. G.J.J. was supported by a Medical Scientist Training Program grant (GM07365) provided by the National Institute of General Medical Science at the National Institutes of Health. This research was supported by National Institutes of Health Grant AI21144 to R.D.K.Additional details
- Eprint ID
- 91764
- Resolver ID
- CaltechAUTHORS:20181213-110052503
- GM07365
- NIH
- AI21144
- NIH
- Created
-
2018-12-13Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field