Published September 1963
| public
Journal Article
The Amino Acid Sequence of the γ Chain of Human Fetal Hemoglobin
Abstract
The 146 amino acid residues of the γ chain of human fetal hemoglobin have been placed in sequence. The fetal hemoglobin for this investigation was isolated chromatographically from umbilical cord blood. The α and γ chains were separated prior to the determination of sequence. For the determination of the sequence, peptides were produced by individual hydrolyses with trypsin, chymotrypsin, or pepsin. The sequence of the individual peptides was determined largely through the application of the Edman degradation. The differences between the γ chains of human fetal hemoglobin and the β chains of human adult hemoglobin are responsible for the differences in the properties of the two molecules.
Additional Information
© 1963 American Chemical Society. Received April 15, 1963. We wish to express our thanks to Dr. D. Armstrong, Dr. M. Lazarus, and Dr. T. Perry through whom some samples of cord blood were obtained. Mr. J. T. Cua and Mr. W. D. Fenninger rendered capable assistance in some of the experiments. This investigation was supported in part by a grant (H-2558) from the National Institutes of Health, United States Public Health Service.Additional details
- Eprint ID
- 91060
- DOI
- 10.1021/bi00905a016
- Resolver ID
- CaltechAUTHORS:20181119-161053839
- H-2558
- NIH
- Created
-
2018-11-20Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field
- Other Numbering System Name
- Caltech Division of Chemistry and Chemical Engineering
- Other Numbering System Identifier
- 2996