Published September 7, 2012
| Supplemental Material + Accepted Version
Journal Article
Open
Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag
Abstract
The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.
Additional Information
© Royal Society of Chemistry 2012. Received 1st May 2012, Accepted 6th July 2012. This work was supported by the National Institutes of Health (U54 GM087519 and RC1GM091804 to V. W. C) and by the start up funds from Columbia University (to W. M.). We thank Dr Steffen Jockusch for experimental assistance. V. W. C. holds patents on the TMP-tag technology, and the technology is licensed and commercialized by Active Motif.Attached Files
Accepted Version - nihms401554.pdf
Supplemental Material - c2cc33133k.pdf
Files
c2cc33133k.pdf
Files
(4.1 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:b835bfed6642647ccfc866945ad5d3f0
|
3.0 MB | Preview Download |
|
md5:47840d99489ce029e173ca6f1c73773e
|
1.1 MB | Preview Download |
Additional details
- PMCID
- PMC3775488
- Eprint ID
- 91017
- Resolver ID
- CaltechAUTHORS:20181119-102040204
- U54 GM087519
- NIH
- RC1 GM091804
- NIH
- Columbia University
- Created
-
2018-11-19Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field