Electronic Resonant Stimulated Raman Scattering Micro-Spectroscopy
Abstract
Recently we have reported electronic pre-resonance stimulated Raman scattering (epr-SRS) microscopy as a powerful technique for super-multiplex imaging (Wei, L.; Nature 2017, 544, 465−470). However, under rigorous electronic resonance, background signal, which mainly originates from pump–probe process, overwhelms the desired vibrational signature of the chromophores. Here we demonstrate electronic resonant stimulated Raman scattering (er-SRS) microspectroscopy and imaging through suppression of electronic background and subsequent retrieval of vibrational peaks. We observed a change of the vibrational band shapes from normal Lorentzian, through dispersive shapes, to inverted Lorentzian as the electronic resonance was approached, in agreement with theoretical prediction. In addition, resonant Raman cross sections have been determined after power-dependence study as well as Raman excitation profile calculation. As large as 10⁻²³ cm² of resonance Raman cross section is estimated in er-SRS, which is about 100 times higher than previously reported in epr-SRS. These results of er-SRS microspectroscopy pave the way for the single-molecule Raman detection and ultrasensitive biological imaging.
Additional Information
© 2018 American Chemical Society. Received: July 22, 2018. Revised: September 8, 2018. Published: September 12, 2018. Published as part of The Journal of Physical Chemistry virtual special issue "Young Scientists". W.M. acknowledges support from the US Army Research Office (W911NF-12-1-0594) and R01 (EB020892) and the Camille and Henry Dreyfus Foundation. The authors declare no competing financial interest.Attached Files
Accepted Version - nihms-1017006.pdf
Supplemental Material - jp8b07037_si_001.pdf
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Additional details
- PMCID
- PMC6425473
- Eprint ID
- 91008
- Resolver ID
- CaltechAUTHORS:20181119-102039172
- W911NF-12-1-0594
- Army Research Office (ARO)
- R01 EB020892
- NIH
- Camille and Henry Dreyfus Foundation
- Created
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2018-11-19Created from EPrint's datestamp field
- Updated
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2021-11-18Created from EPrint's last_modified field