Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the drosophila germ line
Abstract
We have used P-element-mediated transformation to introduce a cloned Drosophila alcohol dehydrogenase (Adh) gene into the germ line of ADH null flies. Six independent transformants expressing ADH were identified by their acquired resistance to ethanol. Each transformant carries a single copy of the cloned Adh gene in a different chromosomal location. Four of the six transformant lines exhibit normal Adh expression by the following criteria: quantitative levels of ADH enzyme activity in larvae and adults; qualitative tissue specificity; the size of stable Adh mRNA; and the characteristic developmental switch in utilization of two different Adh promoters. The remaining two transformants express ADH enzyme activity with the correct tissue specificity, but at a lower level than wild type. These results demonstrate that an 11.8 kb chromosomal fragment containing the Adh gene includes the cis-acting sequences necessary for its correct developmental expression, and that a variety of chromosomal sites permit proper Adh gene function.
Additional Information
© 1983 by MIT. Received 27 May 1983. We wish to acknowledge the excellent technical assistance of Karen Livingstone and Abby Telfer. Tip Benyajati, Nick Spoerel, and Kevin O'Hare generously provided unpublished data. We thank Gerry Rubin and Allan Spradling for providing P element vectors, Bill Sofer for fly stocks, and Nick Spoerel for plasmid subclones. J. W. P. was supported by an American Cancer Society postdoctoral fellowship. This work was funded by a grant from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.Additional details
- Eprint ID
- 90549
- DOI
- 10.1016/0092-8674(83)90136-8
- Resolver ID
- CaltechAUTHORS:20181031-133250264
- American Cancer Society
- NIH
- Created
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2018-11-01Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field