Published November 12, 2007
| public
Journal Article
Engineered Alkane‐Hydroxylating Cytochrome P450_(BM3) Exhibiting Nativelike Catalytic Properties
Abstract
Divide, evolve, and conquer: A domain‐based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole‐cell bioconversions of propane to propanol under mild conditions, using air as oxidant.
Additional Information
© 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: June 15, 2007; Published online: September 20, 2007. This work was supported by a Swiss National Science Foundation fellowship to R.F., a U.S. NSF Fellowship to M.M.C. and by the U.S. Army Research Office, ARO Contract DAAD19‐03‐D‐0004. We thank Dr. Christopher Snow for providing the homology model of P450_(BM3) reductase and Dr. Matthew W. Peters, Dr. Peter Meihnold, and Dr. Marco Landwehr for helpful discussions regarding the biotransformations and for providing access to DasGip fermenter.Additional details
- Alternative title
- Engineered Alkane‐Hydroxylating Cytochrome P450BM3 Exhibiting Nativelike Catalytic Properties
- Eprint ID
- 89917
- DOI
- 10.1002/anie.200702616
- Resolver ID
- CaltechAUTHORS:20180925-122611378
- Swiss National Science Foundation (SNSF)
- NSF
- DAAD19‐03‐D‐0004
- Army Research Office (ARO)
- Created
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2018-09-25Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field