Transcriptome profiling of the cardiac neural crest reveals a critical role for MafB
Abstract
The cardiac neural crest originates in the caudal hindbrain, migrates to the heart, and contributes to septation of the cardiac outflow tract and ventricles, an ability unique to this neural crest subpopulation. Here we have used a FoxD3 neural crest enhancer to isolate a pure population of cardiac neural crest cells for transcriptome analysis. This has led to the identification of transcription factors, signaling receptors/ligands, and cell adhesion molecules upregulated in the early migrating cardiac neural crest. We then functionally tested the role of one of the upregulated transcription factors, MafB, and found that it acts as a regulator of Sox10 expression specifically in the cardiac neural crest. Our results not only reveal the genome-wide profile of early migrating cardiac neural crest cells, but also provide molecular insight into what makes the cardiac neural crest unique.
Additional Information
© 2018 Elsevier. Received 1 April 2018, Revised 5 September 2018, Accepted 15 September 2018, Available online 17 September 2018. We thank Dr. Patrick Charnay for the chicken Krox20 probe and the Caltech Flow Cytometry and Genomics Facilities for technical support. This work was supported by Toyobo Biotechnology Foundation (Japan) postdoctoral fellowship and Japan Society for the Promotion of Science KAKENHI Grant Number JP16K18551 to STM, National Institutes of Health (USA) R01DE024157 and RO1HL140587 to MEB and a postdoctoral fellowship from the Shurl and Kay Curci Foundation (USA) to FMV.Attached Files
Accepted Version - nihms-1508445.pdf
Supplemental Material - 1-s2.0-S0012160618302367-mmc1_lrg.jpg
Supplemental Material - 1-s2.0-S0012160618302367-mmc2_lrg.jpg
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Supplemental Material - 1-s2.0-S0012160618302367-mmc6_lrg.jpg
Supplemental Material - 1-s2.0-S0012160618302367-mmc7.xlsx
Supplemental Material - 1-s2.0-S0012160618302367-mmc8.xlsx
Supplemental Material - 1-s2.0-S0012160618302367-mmc9.xlsx
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Additional details
- PMCID
- PMC6421117
- Eprint ID
- 89682
- DOI
- 10.1016/j.ydbio.2018.09.015
- Resolver ID
- CaltechAUTHORS:20180917-125104544
- Toyobo Biotechnology Foundation
- JP16K18551
- Japan Society for the Promotion of Science (JSPS)
- R01DE024157
- NIH
- R01HL140587
- NIH
- Shurl and Kay Curci Foundation
- Created
-
2018-09-17Created from EPrint's datestamp field
- Updated
-
2022-02-16Created from EPrint's last_modified field