Published 1994
| public
Book Section - Chapter
Metal affinity partitioning
- Others:
- Walter, Harry
- Johansson, Göte
Abstract
The partitioning of biological materials in aqueous two-phase systems, for example, poly(ethylene glycol) (PEG)-dextran (Dx) systems, can be altered in a reproducible and predictable fashion using affinity ligands attached to one of the phase-forming polymers. Metal ion complexes such as copper(II) iminodiacetate [Cu(II)IDA] have several very attractive features as affinity ligands: they are stable under a wide range of solvent conditions and temperatures and are therefore easy to recycle; they can be incorporated into chromatographic supports with very high loading capacities; their interactions with target molecules are reasonably specific and are reversible under mild conditions; and they are inexpensive.
Additional Information
© 1994 by Academic Press, Inc. This research is supported by the National Science Foundation, Grant No. EET-8807351, and a Presidential Young Investigator Award (F.H.A.). F.H.A. gratefully acknowledges a fellowship from the David and Lucile Packard Foundation. D.B. is the recipient of a fellowship from the Irvine Foundation.Additional details
- Eprint ID
- 89081
- Resolver ID
- CaltechAUTHORS:20180823-084045820
- EET-8807351
- NSF
- David and Lucile Packard Foundation
- James Irvine Foundation
- Created
-
2018-08-23Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field
- Series Name
- Methods in Enzymology
- Series Volume or Issue Number
- 228