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Published August 2005 | public
Journal Article

Evolving strategies for enzyme engineering

Abstract

Directed evolution is a common technique to engineer enzymes for a diverse set of applications. Structural information and an understanding of how proteins respond to mutation and recombination are being used to develop improved directed evolution strategies by increasing the probability that mutant sequences have the desired properties. Strategies that target mutagenesis to particular regions of a protein or use recombination to introduce large sequence changes can complement full-gene random mutagenesis and pave the way to achieving ever more ambitious enzyme engineering goals.

Additional Information

© 2005 Elsevier Ltd. Available online 11 July 2005. JDB and MMM are supported by Howard Hughes Medical Institute pre-doctoral fellowships, DM is supported by the Royal Society. FHA acknowledges support from the US National Science Foundation, the Army Research Office (DAAD19-03-D-0004) and the National Institutes of Health (grant R01 GM068664-01).

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023