Published December 1997
| public
Journal Article
Directed evolution of enzyme catalysts
- Creators
- Kuchner, Olga
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Arnold, Frances H.
Chicago
An error occurred while generating the citation.
Abstract
Directed enzyme evolution has emerged in the past few years as a powerful alternative to rational approaches for engineering biocatalysts. Prerequisites for successful directed evolution are functional expression in a suitable microbial host, a rapid screen for the desired feature(s) and a well-thought-out working strategy for navigating protein landscapes. The rapidly growing body of literature on enzyme evolution in vitro includes techniques for creating and searching combinatorial enzyme libraries, as well as several successful examples of different evolutionary strategies being used.
Additional Information
© 1997 Published by Elsevier Ltd. Available online 3 February 1998. Support from the Army Research Office, the Office of Naval Research and the Department of Energy's program in Biological and Chemical Technologies Research is acknowledged. O. Kuchner is the recipient of an NIH predoctoral training fellowship from the National Institute of General Medical Sciences, NRSA Award 1 T32 GM 08346-01. The authors thank Daniel Song for helpful discussions.Additional details
- Eprint ID
- 89068
- Resolver ID
- CaltechAUTHORS:20180822-161250434
- Army Research Office (ARO)
- Office of Naval Research (ONR)
- Department of Energy (DOE)
- NIH Predoctoral Fellowship
- 1 T32 GM 08346-01
- Created
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2018-08-23Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field