Published July 21, 2003
| public
Journal Article
A Self-Sufficient Peroxide-Driven Hydroxylation Biocatalyst
- Creators
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Cirino, Patrick C.
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Arnold, Frances H.
Chicago
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Abstract
Directed evolution of the heme domain of cytochrome P450 BM‐3 has resulted in a versatile, highly active peroxide‐driven hydroxylation catalyst (see picture) that requires neither NADPH nor reductase and functions in a cell‐free reaction system. This simplified, "biomimetic" catalyst is amenable to further optimization, for example, to improve stability or alter its substrate range.
Additional Information
© 2003 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. Received: March 18, 2003. This work was supported by the Biotechnology Research and Development Corporation (Peoria, IL) and an NSF Graduate Research Fellowship awarded to P.C.C. The authors thank Ulrich Schwaneberg for guidance and Denis Shcherbakov for laboratory assistance.Additional details
- Eprint ID
- 89063
- Resolver ID
- CaltechAUTHORS:20180822-145522004
- Biotechnology Research and Development Corporation
- NSF Graduate Research Fellowship
- Created
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2018-08-22Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field